Transport of Bacteria in Porous Media. Part I: An Experimental Investigation

TitleTransport of Bacteria in Porous Media. Part I: An Experimental Investigation
Publication TypeJournal Article
Year of Publication1994
AuthorsGeorgiou, G., A.K. Sarkar, and M. M. Sharma
JournalBiotechnology & Bioengineering Journal
Volume44
Issue04
Start Page489
Pagination489-497
Date Published08/1994
Other Numbers0006-3592/94/040489-9
KeywordsBacterial Adhesion / Plugging, Basic Surface and Colloid Science, Colloid Transport, Formation Damage
Abstract

The convective transport of concentrated suspension of bacteria in porous media is of interest for several processes such as microbial enhanced oil recovery and in situ bioremediation. The parameters which affect the transport of the bacterium Bacillus licheniformis JF-2, a candidate microorganism for microbial enhanced oil recovery, were investigated experimentally in sandpacks. Bacteria retention and permeability reduction occurred primarily in the first few centimeters upon entering the porous medium. In downstream sections of the sandpack, the permeability reduction was low, even in cases in which high cell concentrations (108 cfu/mL) were detected in the effluent. The effect of (i) addition of a dispersant, (ii) linear velocity of injection, (iii) cell concentration, (iv) salinity (v) temperature, and (vi) the presence of a residual oleic phase were determined experimentally. A lower reduction in permeability and a higher effluent bacterial concentration were obtained in the presence of dispersant, high injection velocities, low salinities, and at a higher temperature. Macroscopic measurements at different linear velocities and in the presence or absence of dispersants suggest that the formation of reversible microaggregates and multiparticle hydrodynamic exclusion may be the primary mechanisms for bacterial retention and permeability reduction

DOI10.1002/bit.260440412